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Quercetin induces lipid domain-dependent permeability.

Natália Bueno Leite1, Danubia Batista Martins2, Dayane S Alvares2

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Chemistry and Physics of Lipids
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Summary

Quercetin interacts with cell membranes, altering their properties. It partitions into homogeneous lipid environments but permeates and modifies heterogeneous membranes, impacting cell function.

Keywords:
CholesterolGiant vesicles microscopyLipid bilayer effectsLipid/water partition coefficientsPhospholipid membranesQuercetin

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Area of Science:

  • Biophysics
  • Cell Biology
  • Pharmacology

Background:

  • Quercetin, a polyphenol, exhibits diverse biological activities.
  • Its mechanisms involve interactions with cellular components, often within membrane environments.
  • Understanding quercetin's membrane interactions is crucial for elucidating its biological roles.

Purpose of the Study:

  • To investigate quercetin's interaction with model cell membranes of varying lipid compositions.
  • To determine the influence of lipid phases and bilayer homogeneity on quercetin's action.
  • To explore the biophysical consequences of quercetin-membrane interactions.

Main Methods:

  • Utilized giant unilamellar vesicles (GUVs) and large unilamellar vesicles (LUVs) with diverse lipid compositions.
  • Analyzed quercetin's effects on vesicle morphology, permeability, and rigidity.
  • Assessed quercetin's partitioning and affinity to model membranes.

Main Results:

  • Quercetin preferentially partitions into homogeneous lipid environments.
  • It significantly permeates and modifies heterogeneous membranes containing coexisting liquid-disordered, liquid-ordered, and solid phases.
  • Quercetin rigidifies bilayers with high cholesterol content and causes domain coalescence in sphingomyelin-containing systems, increasing permeability.

Conclusions:

  • Lipid phase behavior and bilayer homogeneity are critical factors in quercetin's membrane interactions.
  • Quercetin's effects vary depending on membrane composition, influencing membrane structure and function.
  • These findings contribute to understanding quercetin's multifaceted biological activities through its membrane-modulating properties.