Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

423
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
423
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.4K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.4K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.5K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.5K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

809
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
809
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

549
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
549
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shrimp TM9SF4 facilitates WSSV infection probably through interacting with viral VP24 and regulating host autophagy.

Fish & shellfish immunology·2026
Same author

Nanoparticles as Innovative Tools for Enhancing Abiotic Stress Tolerance and Supporting Integrated Disease Management in Oil Crops.

Physiologia plantarum·2026
Same author

CRISPR/Cas9-Mediated Editing of Four Ca<sup>2+</sup>-Binding Receptor-Like Cytoplasmic Kinases Improves Soybean Seed Size and Yield at Dense Planting.

Plant biotechnology journal·2026
Same author

Metal nanoparticles enhance ROS scavenging, nitrogen metabolism, and stress-responsive pathways to improve soybean yield and seed quality.

Plant physiology and biochemistry : PPB·2026
Same author

Structural and functional analysis of growth differentiation factor 8: A positive regulator of growth in the Pacific white shrimp Litopenaeus vannamei.

International journal of biological macromolecules·2026
Same author

The Rice Cis-Natural Antisense Transcript NAT1850 of Pri-miR1850 Negatively Regulates Cold Tolerance by Repressing NPR3.

Plant biotechnology journal·2026

Related Experiment Video

Updated: Oct 12, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

A compound post-column re-focusing approach in supercritical fluid chromatography.

Mingzhe Sun1, Peter Schoenmakers1

  • 1Analytical Chemistry Group, Van't Hoff Institute for Molecular Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands; Centre for Analytical Sciences Amsterdam (CASA), The Netherlands.

Journal of Chromatography. A
|November 22, 2021
PubMed
Summary

This study introduces a post-column re-focusing technique for supercritical-fluid chromatography (SFC) to improve detection sensitivity. The method enhances analyte signals and concentrations, overcoming limitations of CO2-based mobile phases.

Keywords:
Concentration enhancementHeart-cutRe-mobilizingSignal enhancementTrapping

More Related Videos

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

6.6K
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.2K

Related Experiment Videos

Last Updated: Oct 12, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K
Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

6.6K
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.2K

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Supercritical-fluid chromatography (SFC) is gaining prominence due to instrumentation advancements.
  • CO2-based mobile phases and high flow rates in SFC pose challenges for trace analysis and detector coupling.

Purpose of the Study:

  • To develop a post-column re-focusing approach for SFC analysis.
  • To achieve signal and concentration enhancement for UV-Vis detection in SFC.

Main Methods:

  • A post-column re-focusing strategy involving heart-cutting fractions into a trapping column.
  • Analyte re-mobilization using a flushing solvent after CO2 elimination.
  • Optimization of trapping stationary phase and solvents for selected compounds.

Main Results:

  • Achieved signal enhancement ratios between 2.2 and 6.4 for four representative compounds.
  • Observed actual concentration enhancement ratios between 1.7 and 2.9.
  • Demonstrated the effectiveness of the method across varying SFC modifier percentages.

Conclusions:

  • The proposed post-column re-focusing technique effectively enhances analyte detection in SFC.
  • This method addresses sensitivity limitations associated with CO2-based SFC.
  • The approach offers a viable solution for trace analysis in SFC applications.