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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Oct 12, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Roadmap on digital holography [Invited].

Bahram Javidi, Artur Carnicer, Arun Anand

|November 23, 2021

Summary

This summary is machine-generated.

This roadmap explores digital holography, covering its progress and challenges in sensing, 3D imaging, microscopy, and virtual reality applications. It highlights key developments and future directions in this dynamic field.

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Last Updated: Oct 12, 2025

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Area of Science:

Optics and Photonics
Digital Imaging
Biomedical Engineering

Background:

Digital holography is a rapidly advancing field with diverse applications.
Expert consensus is crucial for charting future research directions.

Purpose of the Study:

To provide a comprehensive overview of current research in digital holography.
To outline the future vision, progress, and challenges in the field.
To consolidate insights from 25 leading experts in digital holography.

Main Methods:

A compilation of 25 expert-authored sections.
Review of diverse research areas including sensing, 3D imaging, microscopy, and cell analysis.
Synthesis of expert perspectives on progress, impact, and challenges.

Main Results:

Detailed insights into digital holography's role in sensing, 3D imaging, and displays.
Exploration of applications in virtual and augmented reality, and advanced microscopy.
Coverage of cell identification, tomography, and label-free live cell imaging.

Conclusions:

Digital holography is a versatile technology with significant potential across multiple scientific and technological domains.
The field faces challenges that require continued innovation and research.
This roadmap provides a vision for the future development and application of digital holography.