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Surface plasmon resonance aptasensor for Brucella detection in milk.

Ali D Dursun1, Baris A Borsa2, Gulay Bayramoglu3

  • 1Department of Physiology, School of Medicine, Atilim University, 06830, Ankara, Turkey; Vocational School of Health Services, Atilim University, 06830, Ankara, Turkey.

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|November 23, 2021
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Summary

A new aptasensor detects Brucella melitensis (B. melitensis) in milk. This method uses magnetic nanoparticles and Surface Plasmon Resonance (SPR) for rapid, sensitive detection of this foodborne pathogen.

Keywords:
AptamersBrucellaMagnetic microbeadsMilkSPR aptasensor

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Area of Science:

  • Biotechnology
  • Biosensing
  • Food Safety

Background:

  • Brucellosis is a significant zoonotic disease globally, primarily transmitted through contaminated milk and dairy products.
  • Current detection methods for Brucella melitensis (B. melitensis) can be time-consuming and may suffer from matrix interference in complex samples like milk.

Purpose of the Study:

  • To develop a highly sensitive and specific aptasensor for the rapid detection of B. melitensis in raw milk.
  • To integrate magnetic-based pre-concentration with Surface Plasmon Resonance (SPR) detection to overcome limitations of direct milk sample analysis.

Main Methods:

  • Selection of high-affinity and high-specificity DNA aptamers against B. melitensis using whole-cell SELEX.
  • Immobilization of a high-affinity aptamer (B70) onto magnetic silica core-shell nanoparticles for bacterial capture and purification.
  • Preparation of SPR sensor chips functionalized with a specific aptamer (B46) for sensitive detection of eluted bacteria.

Main Results:

  • Two aptamers with excellent affinity and specificity for B. melitensis were successfully selected.
  • An integrated magnetic isolation and SPR detection strategy significantly reduced background noise and improved sensitivity.
  • The developed aptasensor achieved a low limit of detection (LOD) of 27 ± 11 cells in 1 ml of milk, demonstrating high efficiency for B. melitensis contamination detection.

Conclusions:

  • The developed SPR aptasensor, coupled with magnetic nanoparticle-based sample purification, offers a rapid, sensitive, and specific method for detecting B. melitensis in milk.
  • This approach holds significant potential for improving food safety surveillance and preventing the spread of brucellosis.
  • The integrated system provides a promising tool for real-time monitoring of bacterial contamination in the dairy industry.