Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

1.2K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.2K
The Extracellular Matrix01:29

The Extracellular Matrix

9.8K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of small-molecule HSF1 amplifiers by high content screening in protection of cells from stress induced injury.

Biochemical and biophysical research communications·2009
Same author

Nanowire transformation by size-dependent cation exchange reactions.

Nano letters·2009
Same author

Effect of haishengsu as an adjunct therapy for patients with advanced renal cell cancer: a randomized and placebo-controlled clinical trial.

Journal of alternative and complementary medicine (New York, N.Y.)·2009
Same author

Identification of inhibitors of HSF1 functional activity by high-content target-based screening.

Journal of biomolecular screening·2009
Same author

Antitumor effects of targeting hTERT lentivirus-mediated RNA interference against KB cell lines.

Oncology research·2009
Same author

Characteristics of emissive spectrum and the removal of nitric oxide in N2/02/NO plasma with argon additive.

Journal of environmental sciences (China)·2009

Related Experiment Video

Updated: Oct 12, 2025

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.4K

BMSC-derived extracellular matrix better optimizes the microenvironment to support nerve regeneration.

Shengran Wang1, Changlai Zhu1, Bin Zhang1

  • 1Jiangsu Key Laboratory of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, 19 Qixiu Road, Nantong, JS, 226001, PR China.

Biomaterials
|November 23, 2021
PubMed
Summary

Bone marrow mesenchymal cell-derived extracellular matrix (ECM) creates a superior microenvironment for nerve regeneration. This ECM, when used in nerve grafts, significantly enhances nerve repair compared to other cell-derived ECMs.

Keywords:
Extracellular matrixMicroenvironmentNerve regenerationTissue engineering

More Related Videos

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

13.3K
Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.5K

Related Experiment Videos

Last Updated: Oct 12, 2025

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.4K
Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

13.3K
Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.5K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Neuroscience

Background:

  • A conducive microenvironment is crucial for effective nerve regeneration.
  • Extracellular matrix (ECM) components and organization influence nerve repair.
  • Existing ECM sources like Schwann cells (SCs) and fibroblasts (FBs) have limitations.

Purpose of the Study:

  • To compare the nerve regeneration potential of ECMs derived from bone marrow mesenchymal cells (BMSCs) with other cell types.
  • To engineer and evaluate ECM-modified nerve grafts (ECM-NGs) for peripheral nerve defect repair.
  • To elucidate the molecular and structural advantages of BMSC-derived ECMs in promoting nerve regeneration.

Main Methods:

  • Proteomics and 3D image analysis to characterize ECM composition and spatial organization.
  • Engineering of ECM-NGs by co-culturing BMSCs, SCs, SKP-SCs, or FBs with nerve grafts.
  • Histological, neurophysiological, and behavioral analyses to assess nerve repair efficacy.

Main Results:

  • BMSC-derived ECM exhibited greater similarity to acellular nerve ECM compared to SC, SKP-SC, or FB ECM.
  • BMSC-ECM-NGs demonstrated superior nerve regeneration, evidenced by improved histology, neurophysiology, and function.
  • Proteomic analysis revealed enhanced neural regeneration factors and reduced immune response in the BMSC-ECM-NG microenvironment.

Conclusions:

  • BMSC-derived ECM provides a more favorable microenvironment for nerve regeneration than other tested ECMs.
  • BMSC-ECM-NGs represent a promising strategy for peripheral nerve defect repair.
  • This approach offers a potential clinical alternative for enhancing nerve regeneration.