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Related Experiment Videos

Progress on gene transfer in farm animals.

V G Pursel1, C E Rexroad, D J Bolt

  • 1Laboratory of Reproductive Physiology, University of Pennsylvania.

Veterinary Immunology and Immunopathology
|December 1, 1987
PubMed
Summary
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Transgenic pigs and sheep were created by microinjecting growth hormone genes. While growth rates were not enhanced, biological effects like reduced backfat and increased IGF-I were observed in transgenic pigs.

Area of Science:

  • Animal biotechnology
  • Transgenic animal development
  • Gene expression in livestock

Background:

  • Genetic engineering offers potential for livestock improvement.
  • Metallothionein promoter allows for gene expression regulation.
  • Growth hormone genes are key regulators of animal growth and development.

Purpose of the Study:

  • To produce transgenic pigs and sheep using mouse metallothionein promoter fused with human or bovine growth hormone genes.
  • To investigate the integration, expression, and biological effects of these foreign genes in transgenic livestock.
  • To assess the heritability of the introduced gene construct.

Main Methods:

  • Microinjection of single-cell zygotes and two-cell ova with linear DNA molecules containing the MT promoter and GH genes.

Related Experiment Videos

  • Analysis of gene integration into the host genome using Southern blotting or similar techniques.
  • Measurement of growth hormone levels, IGF-I, backfat thickness, and mammary development in transgenic animals.

    • Main Results:

    • Successful integration of foreign genes into pigs and sheep genomes.
    • Expression of human growth hormone (hGH) and bovine growth hormone (bGH) detected in plasma of transgenic animals.
    • Transgenic pigs exhibited reduced backfat, elevated IGF-I, stimulated mammary development, and suppressed endogenous porcine growth hormone (pGH), despite no enhanced growth rate.
    • The MT-hGH gene construct was heritable, with founder pigs transmitting the gene to progeny.

    Conclusions:

    • Transgenic pigs and sheep can be generated with foreign growth hormone genes.
    • The introduced growth hormone genes exert significant biological effects in transgenic pigs, independent of enhanced growth rate.
    • The gene construct demonstrates heritability, enabling potential for further genetic improvement programs in livestock.