Three-dimensional visualization of secondary neurulation in chick embryos using microCT

Area of Science:

• Developmental Biology
• Embryology
• Imaging Science

Background:

• Secondary neurulation defects are linked to neural tube defects.
• Previous research relied on microscopy and molecular methods.
• Histology has limitations in visualizing gross 3D embryonic structures.

Purpose of the Study:

• To visualize secondary neurulation and related structures in chick embryos (HH stages 10-22).
• To overcome the limitations of conventional histology for 3D embryonic morphology.
• To utilize microCT for enhanced visualization of early embryonic development.

Main Methods:

• Micro computed tomography (microCT) scanning of chick embryos.
• Observation across Hamburger and Hamilton (HH) stages 10-22.
• Analysis of gross morphology and detailed structures.

Main Results:

• MicroCT effectively visualized chick embryo gross morphology across developmental stages.
• Detailed structures of the caudal cell mass (CCM) were revealed from HH stage 12 to 16.
• Spatiotemporal relationships, dynamic changes of key structures like the chordoneural hinge, and neural tube cavitation were described.

Conclusions:

• MicroCT offers significant advantages for visualizing secondary neurulation in early chick embryos.
• The study provides a valuable 3D reference for related embryonic structures.
• This technique enhances understanding of developmental processes and potential defects.