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Updated: Oct 12, 2025
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
Derivatizing merocyanine dyes to balance their polarity and viscosity sensitivities for protein aggregation detection
Yulong Bai1,2, Yanan Huang1, Wang Wan1
1CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. liuyu@dicp.ac.cn.
Abstract:
Protein misfolding and aggregation processes involve local polarity and viscosity fluctuation. Herein we modulated the polarity and viscosity sensitivities of merocyanine dyes to detect protein aggregation. We demonstrated how structural modulation balanced these two fluorescence sensitivities and affected the detection of misfolded and aggregated proteins.
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