Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination01:51

Determination

19.6K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
19.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Teeth01:15

Teeth

895
The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
895
Cellular Differentiation00:57

Cellular Differentiation

4.0K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
4.0K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

3.9K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.9K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

8.0K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptome and 16S rRNA Amplicon Sequencing Analysis of Nutrition Metabolism in Silver Pomfret at Varying Flow Rates.

Animals : an open access journal from MDPI·2026
Same author

Food-Derived Antidiabetic Peptides as Multi-Target Systemic Regulators: A Comprehensive Review of Sources, Preparation, Mechanisms and Future Perspectives.

Foods (Basel, Switzerland)·2026
Same author

Corrigendum to 'Identification of cell type specific marker genes of dental epithelial cells using single cell RNA sequence: a Review' [J. Oral Biosci. 68, (2026) 100757].

Journal of oral biosciences·2026
Same author

Case Report: Exploring the link between severe gingivitis and inflammatory bowel disease in a young patient.

Frontiers in dental medicine·2026
Same author

Fibroblast growth factor 8 regulates early Meckel's cartilage development via ERK signaling in a stage- and dose-dependent manner.

Developmental biology·2026
Same author

Prognostic impact of treatment patterns of in-hospital heart failure on clinical outcomes after myocardial infarction.

Coronary artery disease·2026

Related Experiment Video

Updated: Oct 12, 2025

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.9K

The tooth-specific basic helix-loop-helix factor AmeloD promotes differentiation of ameloblasts.

LingLing Jia1,2, Yuta Chiba1,3, Kan Saito1

  • 1Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan.

Journal of Cellular Physiology
|November 23, 2021
PubMed
Summary
This summary is machine-generated.

AmeloD is crucial for tooth enamel formation. Knocking out AmeloD in mice led to abnormal enamel structure and reduced key gene expression, confirming its role in ameloblast differentiation.

Keywords:
ameloblastsbHLH transcription factorcell differentiationknockout micetooth development

More Related Videos

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.3K
Propagation of Dental and Respiratory Cells and Organs in Microgravity
06:29

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

2.2K

Related Experiment Videos

Last Updated: Oct 12, 2025

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.9K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.3K
Propagation of Dental and Respiratory Cells and Organs in Microgravity
06:29

Propagation of Dental and Respiratory Cells and Organs in Microgravity

Published on: May 25, 2021

2.2K

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Tissue-specific basic helix-loop-helix (bHLH) transcription factors regulate cellular differentiation.
  • AmeloD is a newly identified tooth-specific bHLH transcription factor.
  • The specific role of AmeloD in dental cell differentiation remains unexplored.

Purpose of the Study:

  • To investigate the function of AmeloD in dental epithelial cell differentiation.
  • To elucidate the role of AmeloD in the process of amelogenesis.

Main Methods:

  • AmeloD-knockout (AmeloD-KO) mouse model was utilized.
  • Analysis of molar structure and ion composition in AmeloD-KO mice.
  • Gene expression analysis of Sox21 and ameloblast differentiation markers.
  • Overexpression of AmeloD in the M3H1 dental epithelial cell line.

Main Results:

  • AmeloD-KO mice exhibited abnormal molar enamel structure and altered ion composition, indicative of enamel hypoplasia.
  • Downregulation of SRY-Box transcription factor 21 (Sox21) and ameloblast differentiation markers was observed in AmeloD-KO mouse molars.
  • Overexpression of AmeloD in M3H1 cells led to upregulation of Sox21 and ameloblast differentiation markers.

Conclusions:

  • AmeloD is essential for proper amelogenesis and promotes ameloblast differentiation.
  • AmeloD plays a critical role in regulating the expression of Sox21 and other key genes during tooth enamel formation.
  • This study enhances understanding of the molecular mechanisms underlying amelogenesis.