Polymerized phosphonium ionic liquid functionalized silica microspheres as mixed-mode stationary phase for liquid...

There is a large and growing demand for the vigorous development of new high performance liquid chromatography stationary phases in order to solve complex phospholipids separation. Herein, phosphonium-based ionic liquid trioctyl(allyl)phosphonium bromide ([P_888Allyl]Br) was first synthesized with trioctylphosphine and allyl bromide. With [P_888Allyl]Br as the polymerizable monomer, polymerized phosphonium ionic liquid functionalized silica microsphere (PIL@SiO₂) was further synthetized via click chemistry reaction. Significantly, based on the inherent amphiphilic nature of the introduced [P_888Allyl]Br, the packed PIL@SiO₂ column displayed hydrophilic/hydrophobic mixed-mode retention mechanisms. The PIL@SiO₂ column can achieve separation of nucleic acid bases and nucleosides, sulfonamides, amides and anilines with excellent selectivity in a shorter separation time. The column efficiency reached 109,700 N/m for 2-iodoacetamide. One of the important characteristics of the PIL@SiO₂ column is that both phospholipid classes and species can be efficiently separated via the same column, outperforming that of the commercial amino column. Furthermore, the application potential of the PIL@SiO₂ column was further verified via separation of phospholipids extracted from soy lecithin. The proposed PIL@SiO₂ column provides a promising candidate for separation of complex phospholipid samples.