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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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UV–Vis Spectrometers01:14

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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UV–Vis Spectrum01:30

UV–Vis Spectrum

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When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
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IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

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In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
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O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
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Using a simple spectrophotometer to analyze cypress hydrolat composition.

Chang-Lung Yen1, Jian-Hung Chen1, Hung-Yu Chien1

  • 1College of Management, National Chi Nan University, Nantou County 545, Taiwan (R.O.C.).

Mathematical Biosciences and Engineering : MBE
|November 24, 2021
PubMed
Summary
This summary is machine-generated.

This study found that specific wavelengths (205-350 nm) detected by a simple spectrophotometer can accurately measure Taiwan cypress hydrolat concentration. This offers a low-cost quality control method for its bactericidal applications.

Keywords:
GC-MSconcentration measurementcypress hydrolatspectrophotometer

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Area of Science:

  • Analytical Chemistry
  • Natural Product Chemistry

Background:

  • Taiwan cypress hydrolat (Pure Dew) exhibits bactericidal properties.
  • High costs associated with quality control and concentration measurement limit its application.

Purpose of the Study:

  • To identify spectral frequencies for reliable concentration measurement of cypress hydrolat.
  • To develop a cost-effective quality control method using spectrophotometry.

Main Methods:

  • Gas Chromatography-Mass Spectrophotometry (GC-MS) was used to analyze hydrolat composition.
  • Hydrolat samples were diluted to varying concentrations (100, 50, 25, 0% v/v).
  • Spectrophotometry was employed to measure absorbance across different wavelengths.

Main Results:

  • The spectral range of 205-350 nm proved most representative for absorbance detection.
  • A roughly linear relationship was observed between absorbance and cypress hydrolat concentration.
  • Simple spectrophotometry can serve as a viable tool for concentration assessment.

Conclusions:

  • Spectrophotometry offers a low-cost, high-efficiency method for determining Taiwan cypress hydrolat concentration.
  • This facilitates broader application of cypress hydrolat by simplifying quality control.
  • Further development can optimize this technique for industrial use.