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Updated: Oct 12, 2025
A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis
Published on: November 30, 2021
A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
Lila S Nolan1, Qingqing Gong1, Heather N Hofmeister1
1Department of Pediatrics, Division of Newborn Medicine, Washington University School of Medicine, St. Louis Children's Hospital, 660 S. Euclid Avenue, Campus Box 8208, St. Louis, MO 63110, USA.
None:
Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).
