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Long noncoding RNA SNHG3 promotes malignant phenotypes in cervical cancer cells via association with YAP1

Hongyu Zhu1, Chenyu Zhu2, Xiang Feng3

  • 1Gynecology Second Ward, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, 443003, Hubei, China. hongyuzhu@ctgu.edu.cn.

Human Cell
|November 24, 2021
PubMed

Insights

Small Nucleolar RNA Host Gene 3 (SNHG3) promotes cervical cancer (CC) growth and metastasis by interacting with YAP1. Upregulated SNHG3 indicates poor prognosis, suggesting it as a potential therapeutic target for CC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA (LncRNA) Small Nucleolar RNA Host Gene 3 (SNHG3) is implicated in various cancers.
  • The specific role and mechanism of SNHG3 in cervical cancer (CC) remain largely undetermined.

Purpose of the Study:

  • To investigate the expression, prognostic value, and functional role of SNHG3 in cervical cancer.
  • To elucidate the underlying molecular mechanism of SNHG3 in CC progression, particularly its interaction with YAP1.

Main Methods:

  • Quantitative real-time PCR to assess SNHG3 expression in CC tissues.
  • In vitro cell assays (proliferation, migration, invasion) and in vivo tumor growth models.
  • Western blotting and co-immunoprecipitation to study SNHG3-YAP1 interaction and YAP1 degradation.
  • Analysis of YAP1 target gene transcription.

Main Results:

  • SNHG3 expression was significantly upregulated in CC tissues and correlated with advanced FIGO stage, metastasis, and poor patient survival.
  • Overexpression of SNHG3 promoted CC cell proliferation, migration, and invasion in vitro, and enhanced tumor growth in vivo.
  • SNHG3 directly interacted with YAP1, inhibiting its degradation and modulating the transcription of YAP1 target genes. The oncogenic effects of SNHG3 were partly mediated by YAP1.

Conclusions:

  • SNHG3 plays a significant oncogenic role in cervical cancer progression.
  • SNHG3 serves as a potential prognostic biomarker and a promising therapeutic target for cervical cancer.
Keywords:
InvasionMigrationProliferationProtein stability

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