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Thyroid hormones alter mRNA activity profiles of differentiated 3T3-L1-cells
J Clement1, S Hausdorf, F S Keck
1Department of Internal Medicine I, University of Ulm, Germany.
Hormone and Metabolic Research. Supplement Series
|January 1, 1987
Abstract:
The influence of thyroid hormones on the gene expression of 3T3-L1-adipocytes was examined. mRNA activity profiles were investigated by in vitro-translation and 2D-electrophoresis. Seven protein spots out of 210 were sensitive to 3,5,3'-triiodothyronine (T3). All proteins increased from hypothyroid to hyperthyroid status. Additionally two spots increased from hypothyroid to T3-substituted and euthyroid status. There is evidence for the existence of protein S14 in differentiated 3T3-L1-cells, as part of spot 5.