Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

29.6K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
29.6K
Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

27.9K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
27.9K
Introduction to Seed Plants03:40

Introduction to Seed Plants

65.7K
Most plants are seed plants—characterized by seeds, pollen, and reduced gametophytes. Seed plants include gymnosperms and angiosperms.
65.7K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

23.3K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
23.3K
Introduction to Plant Diversity02:22

Introduction to Plant Diversity

46.4K
From Water to Land
46.4K
Seedless Vascular Plants03:24

Seedless Vascular Plants

64.5K
Seedless Vascular Plants Were the First Tall Plants on Earth
64.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Report of <i>Ophiostoma clavatum</i> and <i>Fusarium verticillioides</i> Associated With <i>Ips acuminatus</i>-Infested Scots Pine in Western Ukraine.

Plant-environment interactions (Hoboken, N.J.)·2026
Same author

Isolation and Characterization of Multi-Trait Plant Growth-Promoting Endophytic Bacteria from Scots Pine Tissues.

Journal of microbiology and biotechnology·2025
Same author

DNA binding and transposition activity of the Sleeping Beauty transposase: role of structural stability of the primary DNA-binding domain.

Nucleic acids research·2024
Same author

Translational Diffusion and Self-Association of an Intrinsically Disordered Protein κ-Casein Using NMR with Ultra-High Pulsed-Field Gradient and Time-Resolved FRET.

The journal of physical chemistry. B·2024
Same author

Heterodimers Are an Integral Component of Chemokine Signaling Repertoire.

International journal of molecular sciences·2023
Same author

A new obligate CXCL4-CXCL12 heterodimer for studying chemokine heterodimer activities and mechanisms.

Scientific reports·2022
Same journal

Identification of CAMTA transcription factors and functional analysis of OsCAMTA4 in rice blast and salt stress.

Planta·2026
Same journal

Genetic identification and candidate gene analysis for loci of spike density in wheat.

Planta·2026
Same journal

Regulatory roles of R2R3-MYB genes in plant growth, development and stress adaptation: insights into seed dormancy and germination.

Planta·2026
Same journal

Assembly and comparative analysis of the complete mitochondrial genome of Viola philippica (Malpighiales, Violaceae).

Planta·2026
Same journal

Somatic embryogenesis-induced epigenetic changes promoting catechin accumulation in Vaccinium vitis-idaea L.

Planta·2026
Same journal

Integrative transcriptome and long non-coding RNA analysis to decipher the molecular basis of cleistogamy in pigeonpea (Cajanus cajan (L) Millsp).

Planta·2026
See all related articles

Related Experiment Video

Updated: Oct 12, 2025

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.2K

Seed-derived defensins from Scots pine: structural and functional features.

Yulia I Shalovylo1, Yurii M Yusypovych1, Nataliya I Hrunyk1

  • 1Ukrainian National Forestry University, 103, Gen. Chuprynka, St., Lviv, 79057, Ukraine.

Planta
|November 24, 2021
PubMed
Summary
This summary is machine-generated.

Scots pine defensins, PsDef5.1 and PsDef5.2, show broad-spectrum antimicrobial activity against fungi, bacteria, and Candida albicans. These novel plant defensins are promising for agricultural and medical applications.

Keywords:
Antifungal and antibacterial activitiesAntimicrobial peptideCloningExpression patternHeterological expressionPinus sylvestris L.

More Related Videos

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

7.2K
Author Spotlight: In Vitro Co-Culture System of Pine Shoots and Pinewood Nematode for Studying Host Volatile Response
08:42

Author Spotlight: In Vitro Co-Culture System of Pine Shoots and Pinewood Nematode for Studying Host Volatile Response

Published on: September 27, 2024

905

Related Experiment Videos

Last Updated: Oct 12, 2025

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
08:39

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Published on: December 24, 2017

7.2K
Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

7.2K
Author Spotlight: In Vitro Co-Culture System of Pine Shoots and Pinewood Nematode for Studying Host Volatile Response
08:42

Author Spotlight: In Vitro Co-Culture System of Pine Shoots and Pinewood Nematode for Studying Host Volatile Response

Published on: September 27, 2024

905

Area of Science:

  • Plant Science
  • Molecular Biology
  • Biochemistry

Background:

  • Plant defensins are ubiquitous antimicrobial peptides with diverse functions.
  • Most characterized defensins originate from angiosperms; gymnosperm defensins remain largely unstudied.
  • Defensins are encoded by multigenic families and expressed across plant organs.

Purpose of the Study:

  • To isolate and characterize novel seed-derived defensins from Scots pine (gymnosperm).
  • To investigate the antimicrobial activity of a recombinant Scots pine defensin (PsDef5.1).
  • To analyze the expression patterns of new Scots pine defensin genes.

Main Methods:

  • Isolation of cDNA sequences (PsDef5.1 and PsDef5.2) from Scots pine seeds.
  • Expression of mature PsDef5.1 in a prokaryotic system and purification of the recombinant peptide.
  • Antimicrobial assays against phytopathogenic fungi, bacteria, and Candida albicans.
  • Gene expression analysis using transcript detection in various tissues and developmental stages.

Main Results:

  • Two novel cDNA sequences, PsDef5.1 and PsDef5.2, were identified with typical plant defensin features.
  • Recombinant PsDef5.1 demonstrated potent activity against fungi, Gram-positive/negative bacteria (IC50 5-18 µM), and Candida albicans (IC50 6.0 µM).
  • PsDef5.1 and PsDef5.2 transcripts were detected in seeds and seedlings; PsDef5.1 was also found in reproductive organs.

Conclusions:

  • Scots pine defensins exhibit broad-spectrum antimicrobial properties, including against human pathogens.
  • The identified defensins are tissue-specific and developmentally regulated.
  • These novel defensins hold potential for agricultural biotechnology (transgenic applications) and novel antimicrobial drug development.