Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

37.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
37.7K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.5K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.2K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.2K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.0K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.0K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

10.2K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.2K
The Replisome03:01

The Replisome

35.9K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
35.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oral ceramide attenuates ultraviolet Binduced epidermal dysregulation and hyperpigmentation by modulating MITF signaling and barrier-related pathways.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Soy lysolecithin attenuates hypertension and behavioral impairments in mice fed a high-salt diet through receptor-specific regulation of prostaglandin signaling and arachidonic acid-derived prostaglandin production.

Neurochemistry international·2026
Same author

scRepli-RamDA-seq: a multi-omics technology enabling the analysis of gene expression dynamics during S-phase.

Nature communications·2025
Same author

Trisomic rescue via allele-specific multiple chromosome cleavage using CRISPR-Cas9 in trisomy 21 cells.

PNAS nexus·2025
Same author

αvβ3 integrin-targeted magnetic resonance imaging in a pancreatic cancer mouse model using RGD-modified liposomes encapsulated with Fe-deferoxamine.

PloS one·2024
Same author

Booster vaccination using bivalent DS-5670a/b is safe and immunogenic against SARS-CoV-2 variants in children aged 5-11 years: a phase 2/3, randomized, active-controlled study.

Frontiers in immunology·2024
Same journal

Genomic Organization of Ribosomal DNA and Karyotypic Diversity in Vicia sativa and Vicia villosa.

Cytogenetic and genome research·2026
Same journal

George Martin and Werner's Syndrome.

Cytogenetic and genome research·2026
Same journal

The Spectrum of Mosaic Double Aneuploidy of Monosomy X and Trisomy 18: Two New Cases and Review of the Literature.

Cytogenetic and genome research·2026
Same journal

Familial Robertsonian Translocation, rob(14;21), with High Risk for Down Syndrome.

Cytogenetic and genome research·2026
Same journal

Radiosensitivity and Bystander Response in X-Ray-Irradiated Tumour and Normal Epithelial Cells of Breast and Prostate Origin.

Cytogenetic and genome research·2026
Same journal

Cytogenetic Profile of Acute Lymphoblastic Leukaemia in South India: A Series of 1,819 Patients from a Single Centre.

Cytogenetic and genome research·2026
See all related articles

Related Experiment Video

Updated: Oct 12, 2025

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.2K

Camptothecin-Induced Replication Stress Affects DNA Replication Profiling by E/L Repli-Seq.

Takuya Hayakawa1,2,3, Rino Suzuki1, Kazuhiro Kagotani2,3

  • 1Laboratory of Molecular and Cellular Biology, Graduate School of Bioresources, Mie University, Tsu, Japan.

Cytogenetic and Genome Research
|November 24, 2021
PubMed
Summary
This summary is machine-generated.

E/L Repli-seq effectively identifies altered DNA replication programs in cells experiencing replication stress. This method reveals characteristic changes in replication timing domains, aiding in the study of DNA replication under stress.

Keywords:
DNA replicationE/L Repli-seqReplication stressReplication timing domain

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

532
Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

10.8K

Related Experiment Videos

Last Updated: Oct 12, 2025

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.2K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

532
Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

10.8K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • E/L Repli-seq is a tool for mapping DNA replication timing in mammalian cells.
  • Its utility in monitoring DNA replication under stress is not fully understood.

Purpose of the Study:

  • To investigate the application of E/L Repli-seq for analyzing DNA replication under replication stress.
  • To examine temporal DNA replication patterns in human cells treated with camptothecin.

Main Methods:

  • Utilized E/L Repli-seq technology.
  • Treated human retinal pigment epithelium cells with camptothecin, a topoisomerase I inhibitor.
  • Analyzed DNA replication profiles to identify changes in replication timing.

Main Results:

  • E/L Repli-seq profiles showed distinct patterns upon replication stress induction.
  • Observed loss of initiation zones within early replication timing domains.
  • Detected global disappearance of replication timing domain structures, attributed to checkpoint-mediated suppression of initiation.

Conclusions:

  • E/L Repli-seq is effective in identifying cells with altered DNA replication programs due to replication stress.
  • The method can reveal checkpoint-dependent suppression of DNA replication initiation.
  • Demonstrates E/L Repli-seq's capability to study DNA replication dynamics under stress conditions.