Related Experiment Video
Updated: Oct 12, 2025

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Published on: August 25, 2019
Live Birth with or without Preimplantation Genetic Testing for Aneuploidy
Junhao Yan1, Yingying Qin1, Han Zhao1
1From the Center for Reproductive Medicine, Cheeloo College of Medicine, Key Laboratory of Reproductive Endocrinology of the Ministry of Education, and the National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Shandong Key Laboratory of Reproductive Medicine, and Shandong Provincial Clinical Research Center for Reproductive Health, Jinan (J.Y., Y.Q., H.Z., D.W., J.L., T.N., W.Z., K.W., Y.G., Y.S., Z.-J.C.), the Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics (Y.S., Z.-J.C., Y.L., T.Z.), and the Obstetrics and Gynecology Hospital of Fudan University, Shanghai JIAI Genetics and IVF Institute, Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital of Fudan University (X.S., J.F.), Shanghai, the Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, and Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha (F.G., H.M.), the Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, National Clinical Research Center for Obstetrics and Gynecology, Key Laboratory of Assisted Reproduction, Ministry of Education, and Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing (R.L.), the Department of Reproductive Medicine, the Affiliated Obstetrics and Gynecology Hospital with Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital (X.L., J.Z.), and the Department of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University-Jiangsu Province Hospital (X.M., W.W.), Nanjing, the Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou (H.L., Q.M.), the Center for Reproductive Medicine of Yantai Yuhuangding Hospital, Yantai (C.H.), the Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodeling of Liaoning Province, Shenyang (J.T.), the Center for Reproductive Medicine, Wuhan University, Wuhan (J.Y.), the Department of Reproductive Endocrinology, Key Laboratory of Reproductive Genetics, Ministry of Education, Women's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, Zhejiang University School of Medicine, Hangzhou (Y.Z.), the Department of Reproductive Health and Infertility, Guangdong Women and Children Hospital, Guangzhou (F.L.), and the Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei (D.C.) - all in China; the Department of Biostatistics, Yale University School of Public Health, New Haven, CT (H.Z.); and the Department of Obstetrics and Gynecology, Penn State College of Medicine, Hershey, PA (R.S.L.).
Preimplantation genetic testing for aneuploidy (PGT-A) did not significantly improve cumulative live-birth rates compared to conventional in vitro fertilization (IVF). Conventional IVF was found to be noninferior to PGT-A in achieving live births in women with multiple good-quality blastocysts.
Area of Science:
- Reproductive Medicine
- Genetics
- In Vitro Fertilization
Background:
- Preimplantation genetic testing for aneuploidy (PGT-A) is a technique used in IVF to select embryos with a normal number of chromosomes.
- While PGT-A may enhance initial embryo transfer success, its impact on cumulative live-birth rates compared to conventional IVF is not well-established.
Purpose of the Study:
- To compare the cumulative live-birth rate between PGT-A and conventional IVF in subfertile women with at least three good-quality blastocysts.
- To determine if PGT-A is noninferior to conventional IVF in achieving live births.
Main Methods:
- A multicenter, randomized controlled trial involving 1212 subfertile women aged 20-37.
- Participants were assigned to either PGT-A (embryo screening via next-generation sequencing) or conventional IVF (embryo selection by morphology).
- Up to three blastocysts were transferred sequentially, with the primary outcome being the cumulative live-birth rate within one year.
Main Results:
- Live births occurred in 77.2% of the PGT-A group and 81.8% of the conventional IVF group (absolute difference: -4.6 percentage points).
- Conventional IVF demonstrated a noninferior cumulative live-birth rate compared to PGT-A.
- Cumulative clinical pregnancy loss was lower in the PGT-A group (-3.9 percentage points), and obstetrical/neonatal complications were similar between groups.
Conclusions:
- Conventional IVF is noninferior to PGT-A for achieving cumulative live-birth rates in women with multiple good-quality blastocysts.
- The study provides evidence that PGT-A does not offer a significant advantage in overall live-birth success over conventional IVF in this patient population.
More Related Videos
12:32Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
09:30Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Published on: August 17, 2022
Related Concept Videos
Nondisjunction
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Teratogenicity