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Updated: Oct 12, 2025

Predictive Immune Modeling of Solid Tumors

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

Immuno-transcriptomic profiling of extracranial pediatric solid malignancies.

Andrew S Brohl¹, Sivasish Sindiri², Jun S Wei²

¹Sarcoma Department, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA.

|November 24, 2021

Summary

This summary is machine-generated.

This study analyzed pediatric solid tumors to map immune cell infiltration and T-cell receptor diversity. Findings reveal immune profiles predict survival in osteosarcoma and identify new targets for pediatric cancer immunotherapy.

Keywords:

PRAME RNA sequencing T cell receptor adoptive cell therapy immunogenomics immunopeptidomics pediatric oncology tumor-infiltrating lymphocytes

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Area of Science:

Immunogenomics
Pediatric Oncology
Cancer Immunology

Background:

Pediatric extracranial solid tumors are diverse, with varying immune microenvironments.
Understanding immune cell infiltration and T-cell receptor (TCR) dynamics is crucial for developing effective immunotherapies.

Purpose of the Study:

To conduct an immunogenomics analysis of pediatric extracranial solid tumors.
To characterize immune cell patterns, TCR clonal expansion, and immune checkpoints.
To identify novel immunotherapeutic targets for pediatric cancers.

Main Methods:

Whole-transcriptome sequencing of 657 pediatric cancer samples and 131 cell lines.
Comparative analysis with 147 normal tissue samples.
Orthogonal immunopeptidomics approach for target validation.

Main Results:

Significant variation in tumor-infiltrating lymphocytes and TCR counts across and within pediatric cancer types.
TCR counts are predictive of survival in osteosarcoma.
Identification of potential immunotherapeutic targets, including PRAME, across multiple pediatric cancers.

Conclusions:

Immune profiles offer prognostic value in pediatric cancers like osteosarcoma.
Parallel immuno-transcriptomic and immunopeptidomic approaches provide a framework for targeting extracranial solid tumors.
PRAME is validated as a multi-pediatric cancer immunotherapeutic target.