Related Experiment Video

Updated: Oct 12, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.9K

Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A

Zi-Qian Liang1, Li Gao1, Jun-Hong Chen2

  • 1Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, No. 6. Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, China.

International Journal of Genomics
|November 25, 2021
PubMed
Abstract

Insights

Coiled-coil domain containing 80 (CCDC80) is downregulated in ovarian carcinoma (OVCA), suggesting its potential role as a tumor suppressor. Further research is needed to understand its molecular mechanisms and therapeutic implications in OVCA.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian carcinoma (OVCA) is a significant global health concern.
  • Understanding the molecular mechanisms of OVCA is crucial for developing effective treatments.
  • The role of coiled-coil domain containing 80 (CCDC80) in OVCA remains largely unexplored.

Purpose of the Study:

  • To investigate the expression status of CCDC80 in OVCA.
  • To elucidate the underlying molecular mechanisms associated with CCDC80 downregulation in OVCA.
  • To explore the relationship between CCDC80, the tumor microenvironment (TME), and drug sensitivity in OVCA.

Main Methods:

  • Immunohistochemical staining to assess CCDC80 protein expression.
  • Analysis of high-throughput datasets and in-house tissue microarrays for CCDC80 expression levels.
  • Application of CIBERSORT and ssGSEA algorithms to explore CCDC80's correlation with TME components.
  • Pathway enrichment, function annotation, and transcription factor (TF) analysis to identify molecular mechanisms.
  • Utilizing the Genomics of Drug Sensitivity in Cancer (GDSC) database to assess CCDC80's impact on drug sensitivity.

Main Results:

  • CCDC80 was found to be significantly downregulated in OVCA (SMD = -0.919, P = 0.002) with moderate discriminatory ability (AUC = 0.76).
  • Significant differences in naive B cell infiltration were observed between high- and low-CCDC80 expression groups.
  • CCDC80-related genes are enriched in the Ras signaling pathway and lipid metabolism.
  • Nuclear receptor subfamily 3 group C member 1 (NR3C1) may act as an upstream TF for CCDC80.
  • CCDC80 expression may influence sensitivity to mitomycin C and nilotinib.

Conclusions:

  • CCDC80 is downregulated in OVCA, indicating its potential role as a tumor suppressor.
  • CCDC80 may influence the tumor microenvironment, particularly B cell infiltration.
  • CCDC80's molecular pathways and its association with drug sensitivity warrant further investigation for therapeutic strategies in OVCA.

More Related Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.8K
Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence08:55

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Published on: November 2, 2014

12.4K

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.3K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.7K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
9.2K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.6K