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A fluorescence aptasensor based on carbon quantum dots and magnetic Fe3O4 nanoparticles for highly sensitive
Qingyi Wei1, Peiyao Zhang1, Hongbin Pu1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
Abstract:
Trace amounts of 17β-estradiol (E2) in food and the environment poses a threat to human health, which has created the demand for sensitive analytical methods to detect E2. In this study, a novel fluorescent aptasensor was developed for sensitive detection of E2 based on double-chain hybridization between carbon quantum dots-labelled with E2 aptamer (CQDs-aptamer) and Fe3O4 nanoparticles modified by complementary DNA (Fe3O4-cDNA). Under the optimal conditions, the aptasensor displayed a good linear range of 10-11-10-6 M for E2 with the coefficient of determination (R2) of 0.996, and a low detection limit of 3.48 × 10-12 M was obtained. Besides, the aptasensor showed high selectivity and good reproducibility for E2 detection, which was successfully applied to the sensitive detection of E2 in milk as compared with tap water and lake water with satisfactory recoveries from 85.21% to 114.80%, suggesting the great significance of this aptasensor for detecting food contaminants in the food industry.
