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Peptide and ester synthesis in organic solvents catalyzed by seryl proteases linked to alumina
M Pugnière1, A Skalli, M A Coletti-Previero
1Centre de Recherche, INSERM (U 58), Montpellier, France.
Proteins
|October 1, 1986
Abstract:
Trypsin and alpha-chymotrypsin were immobilized to alumina-phosphocolamine complex, activated by glutaraldehyde. The immobilized enzymes show a great stability toward organic solvents miscible or immiscible with water. In the presence of a low concentration of water, the immobilized enzymes catalyzed transesterification reactions as well as peptide synthesis. The synthesized peptides were stable toward the immobilized enzymes.