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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

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Protein scaffolds: A tool for multi-enzyme assembly.

Shubhada Gad¹, Sonal Ayakar¹

¹Department of Biotechnology, Institute of Chemical Technology - IndianOil Odisha Campus Bhubaneswar, Odisha 751013, India.

Biotechnology Reports (Amsterdam, Netherlands)

|November 26, 2021

Summary

Protein scaffolds enhance multi-enzyme systems for complex molecule synthesis. These engineered protein complexes improve enzyme performance and stability, advancing bioprocess technology and bioengineering applications.

Keywords:

Binding modules Dockerin-cohesin interactions Multi-enzyme complex Protein scaffolds SpyTag-SpyCatcher system

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Area of Science:

Biotechnology
Bioprocess Technology
Molecular Engineering

Background:

Simultaneous enzymatic synthesis of complex molecules offers a novel bioprocess technology approach.
Challenges exist in optimizing the operational performance of multiple enzymes within a single reaction vessel.

Purpose of the Study:

To review the role of protein scaffolds in constructing multi-enzyme complexes.
To explore various protein scaffold components and construction strategies.
To discuss the impact of these complexes on enzyme kinetics and applications.

Main Methods:

Review of literature on protein scaffold design and engineering.
Analysis of different enzyme conjugation strategies (e.g., dockerin-cohesin, SpyTag-SpyCatcher, peptide linkers, affibody, sortase).
Examination of analytical and characterization tools for scaffold development.

Main Results:

Protein scaffolds bring enzymes together, enhancing catalytic performance and stability.
Scaffolding creates optimal micro-environments for biochemical reactions.
Various conjugation strategies offer modular approaches to complex assembly.

Conclusions:

Protein scaffold-based mega-enzyme complexes are crucial for advancing bioprocess technology.
These engineered systems show significant promise for diverse biotechnology and bioengineering applications.