Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.6K
Photoluminescence: Applications01:14

Photoluminescence: Applications

545
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
545
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.4K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.4K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

640
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
640
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.9K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

899
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
899

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cover Cropping-Mediated Regulation of Phyllosphere Microbial Health and Rice Yield.

Journal of agricultural and food chemistry·2026
Same author

Brain microstructural alterations on quantitative synthetic MRI and correlation with cognitive dysfunction in patients with β-thalassemia major.

Brain research bulletin·2026
Same author

Polarization-independent dielectric gradient near-perfect absorbers for aqueous mid-infrared molecular sensing.

Npj nanophotonics·2026
Same author

Self-Activated CuAg Metal Heterojunctions for Hydrazine-Assisted Hydrogen Evolution and Zn-Hz Battery Applications.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Aberrant functional connectivity and Granger causality analysis in patients with β-thalassemia major.

Annals of hematology·2026
Same author

Near-Unity-Efficiency Charge Transfer and Quantum Confinement-Regulated Long-Lifetime Charge Recombination in 2D Perovskite/Atomically Thin Semiconductor Heterostructures.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Oct 12, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

549

Polarity-based fluorescence probes: properties and applications.

Xiaojun Qin1,2, Xingye Yang2, Lupei Du2

  • 1School of Pharmacy, Guangxi Medical University Nanning Guangxi 530021 China.

RSC Medicinal Chemistry
|November 26, 2021
PubMed
Summary

Monitoring local polarity is crucial for understanding biological processes and diagnosing diseases. This review highlights polarity-sensitive fluorescent probes for real-time, in vivo detection, aiding research and medical diagnostics.

More Related Videos

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

561
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.4K

Related Experiment Videos

Last Updated: Oct 12, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

549
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

561
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.4K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • Local polarity influences molecular behavior and cellular status.
  • Altered cell polarity is linked to functional disorders and diseases.
  • Accurate polarity detection is vital for biological process elucidation and disease diagnosis.

Purpose of the Study:

  • To review the properties and applications of polarity-sensitive fluorescent probes.
  • To highlight the significance of these probes in biological research.
  • To discuss their role in disease diagnosis.

Main Methods:

  • Review of scientific literature on polarity-sensitive fluorescent probes.
  • Analysis of fluorescence properties and in vivo applications.
  • Summarization of representative probe examples.

Main Results:

  • Several polarity-sensitive fluorescent probes offer safe and real-time in vivo monitoring.
  • These probes exhibit diverse fluorescence properties suitable for various applications.
  • Representative probes demonstrate utility in biological research.

Conclusions:

  • Polarity-sensitive fluorescent probes are valuable tools for biological research.
  • Their in vivo capabilities facilitate real-time monitoring of cellular polarity.
  • These probes hold promise for advancing disease diagnosis and understanding biological mechanisms.