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Updated: Oct 12, 2025
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Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products
Published on: April 6, 2015
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A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection
Gabriel A Russell1,2, Garrie Peng1,2, Cynthia Faubert3
1Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
STAR Protocols
|November 26, 2021
Abstract:
The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
