Progress on the reaction-based methods for detection of endogenous hydrogen sulfide

Area of Science:

• Biochemistry
• Physiology
• Pathology

Background:

• Hydrogen sulfide (H₂S) is a critical endogenous signaling molecule.
• H₂S regulates numerous physiological functions.
• Dysregulation of H₂S is implicated in various pathological conditions.

Purpose of the Study:

• To review current methods for detecting endogenous H₂S.
• To discuss the reaction types, mechanisms, and applications of H₂S detection.
• To provide guidelines for researchers in the field.

Main Methods:

• Review of existing literature on H₂S detection methods.
• Analysis of reactions utilized in H₂S measurement.
• Evaluation of specificity and sensitivity of different assays.

Main Results:

• Various detection methods for H₂S exist, leveraging H₂S-involved reactions.
• These methods offer good specificity and high sensitivity for endogenous H₂S.
• The review categorizes methods based on reaction principles.

Conclusions:

• Accurate measurement of H₂S is vital for understanding its physiological and pathophysiological roles.
• The reviewed methods provide valuable tools for biological research.
• This review aims to guide future research in H₂S detection and application.