Green synthesis, characterization of silver nanoparticals for biomedical application and environmental remediation

Production of silver nanoparticles (Ag-NPs) to increase photocatalytic activity of commercial TiO₂ (P25) and antibacterial activity of surgical sutures was studied. А new method of "green" synthesis of Ag-NPs from aqueous extract of grape skin (oxidation product), which is pre-processed by oxygen and ultrasound is reported. Also, a new method of electrochemical modification of surgical sutures was used. Characterization of Ag-NPs was carried out using energy dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM) and UV-visible spectroscopy. Zeta-potential of obtained colloidal solutions indicated the moderate stability of synthesized nanoparticles. The X-ray powder diffraction (XRD) analysis confirmed the crystallographic structure of the synthesized Ag-NPs. The component profile of grape skin extract has been analyzed using HPLC coupled to diode-array detection and tandem mass spectrometry (HPLC-DAD-MS/MS). In this study absorbable sutures were functionalized with biosynthesized AgNPs through an electrochemical and chemical deposition. Morphological analysis of Ag-NPs-coated surgical sutures was performed by SEM and Energy Dispersive X-Ray Spectroscopy (SEM-EDX) in order to evaluate the presence and distribution of silver deposited on the sutures. The sutures demonstrated bacteriostatic and antifungal effects on Gram-positive (Bacillus subtilis), Gram-negative (Escherichia coli) and Candida albicans wound pathogens. The study revealed that electrochemical deposition of Ag-NPs on nylon surgical sutures did not alter the mechanical properties of the sutures but conferred antibacterial properties. The modified TiO₂ powders with biosynthesized Ag-NPs were characterized by XRD pattern, SEM, their photocatalytic properties, and their antibacterial activities were studied. The results of the antibacterial activity studies showed that TiO₂ modified using green approach possessed higher antibacterial activity against Gram-negative bacteria in comparison with TiO₂ modified by the impregnation method.