Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation

Area of Science:

• Microbiology
• Oral health
• Biotechnology

Background:

• Oral microbiome dysbiosis is linked to human health and disease.
• Photodynamic therapy impacts microbiome diversity, but violet light effects are understudied.

Purpose of the Study:

• To investigate the impact of violet light-emitting diode (LED) irradiation on interdental plaque microbiota.
• To explore violet LED irradiation as a method for modulating oral microbiota balance.

Main Methods:

• Collected interdental plaque from 12 human subjects.
• Exposed plaque samples to violet LED irradiation in a specialized medium.
• Analyzed microbial composition using 16S ribosomal RNA gene sequencing.

Main Results:

• Violet LED irradiation decreased alpha-diversity and altered beta-diversity of the oral microbiota.
• Significant shifts in operational taxonomic units were observed.
• Reduced relative abundance of *Fusobacterium* and increased abundance of *Veillonella* and *Campylobacter* species.

Conclusions:

• Violet LED irradiation modifies oral plaque microbiota composition.
• This method shows potential for adjusting oral microbiome balance without antibiotic resistance.
• Further research can explore therapeutic applications of violet LED irradiation in oral care.