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Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of Acholeplasma laidlawii

María M Tavío1,2, Ana S Ramírez2, Carlos Poveda2

  • 1Microbiología, Facultad de Ciencias de la Salud, Universidad de Las Palmas de Gran Canaria, 35016 Las Palmas, Spain.

Antibiotics (Basel, Switzerland)
|November 27, 2021
PubMed

Insights

Acholeplasma laidlawii exhibits resistance to macrolides, tiamulin, and lincomycin. Multiple mutations in ribosomal proteins L22 and L4, along with 23S rRNA substitutions, drive this antimicrobial resistance.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Biology

Background:

  • Acholeplasma laidlawii is an opportunistic pathogen known to spread antibiotic resistance genes.
  • Macrolide resistance in A. laidlawii is less understood compared to other antibiotic classes.

Purpose of the Study:

  • To investigate the resistance mechanisms against macrolides, tiamulin, and lincomycin in an A. laidlawii strain from a pig with pneumonia.
  • To characterize genetic mutations contributing to antimicrobial resistance in this strain.

Main Methods:

  • Minimum Inhibitory Concentrations (MICs) were determined using microdilution with and without reserpine (an ABC efflux pump inhibitor).
  • Genomic regions, including 23S rRNA and ribosomal proteins L4 and L22, were sequenced.

Main Results:

  • Reserpine affected only lincomycin MICs, not those of macrolides or tiamulin.
  • Eight nucleotide substitutions were found in 23S rRNA, none at known macrolide resistance sites.
  • Five mutations were identified in ribosomal protein L22 and two in L4.

Conclusions:

  • Combined mutations in ribosomal proteins L4, L22, and 23S rRNA substitutions are associated with resistance to macrolides, tiamulin, and lincomycin in A. laidlawii.
  • These findings elucidate novel resistance mechanisms in this important veterinary pathogen.
Keywords:
Acholeplasma laidlawiiantimicrobial resistancelincomycinmacrolidetiamulin

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