Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples

Area of Science:

• Orthopedics
• Molecular Biology
• Genetics

Background:

• Trigger finger is a common fibroproliferative disorder impacting hand function.
• The molecular pathways driving trigger finger fibrosis remain largely unknown.
• Previous research has not explored the complete gene expression profile of trigger finger.

Purpose of the Study:

• To investigate the molecular signaling pathways involved in trigger finger pathogenesis.
• To identify differentially regulated genes and signaling pathways in trigger finger tissue.
• To compare gene expression profiles between trigger finger and carpal tunnel tissues.

Main Methods:

• Utilized the Nanostring nCounter gene expression assay for comprehensive analysis.
• Collected tissue samples from patients undergoing trigger finger release surgery (n=4).
• Compared gene expression profiles with healthy carpal tunnel tissue samples (n=4).

Main Results:

• Identified 165 differentially regulated genes (145 upregulated, 20 downregulated).
• Observed significant upregulation of collagen genes and downregulation of MMP-3.
• Bioinformatic analysis revealed dysregulation in TGF-β1, Wnt, PI3K, MAPK, JAK-STAT, and Notch signaling pathways.

Conclusions:

• The study elucidates novel and known molecular signaling pathways implicated in trigger finger pathogenesis.
• Findings provide a foundation for understanding the molecular basis of trigger finger fibrosis.
• This research offers potential targets for future therapeutic interventions for trigger finger.