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Related Experiment Video

Updated: Oct 12, 2025

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM).

Lukas F Reissig¹, Stefan H Geyer¹, Julia Rose¹

¹Division of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, Austria.

|November 27, 2021

Summary

This summary is machine-generated.

High resolution episcopic microscopy (HREM) generates digital embryo data. This study defines HREM artifacts, crucial for accurately diagnosing developmental disorders in mouse models.

Keywords:

HREM artefacts block face imaging embryo genetically engineered histology mouse embryo

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Area of Science:

Developmental Biology
Microscopy Techniques
Bioimaging

Background:

High resolution episcopic microscopy (HREM) is a powerful technique for generating 3D digital volume data of biological specimens.
Accurate interpretation of HREM data is essential for studying embryonic development and disease models.

Purpose of the Study:

To systematically identify and characterize artifacts in HREM data of mouse embryos.
To assess the impact of these artifacts on the interpretation of wildtype and mutant phenotypes.
To provide guidance for optimizing HREM specimen preparation and data analysis.

Main Methods:

Examination of 607 HREM datasets from mouse embryos (E14.5), including wildtypes and mutants.
Systematic analysis of digital images (2000-4000 per dataset) with voxel dimensions of 3 × 3 × 3 µm³.
Utilized 3D volume models and virtual resections to identify and classify artifacts.

Main Results:

A comprehensive spectrum of characteristic HREM artifacts was identified and categorized by causality.
Specific artifacts were highlighted for their potential to mimic genuine tissue defects and structural pathologies.
Examples were provided to illustrate how artifacts can lead to misinterpretation of embryonic phenotypes.

Conclusions:

Understanding HREM artifacts is vital for accurate phenotyping of mouse embryos.
Distinguishing artifacts from true pathologies ensures correct diagnosis of developmental disorders.
Optimized HREM protocols and data interpretation aid in deciphering mechanisms of developmental disorders.