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Updated: Oct 12, 2025

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

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Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces

Yuqi Lin1, Li Huang1, Xiaoyong Zhang2

  • 1Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

International Journal of Molecular Sciences
|November 27, 2021
PubMed

Insights

Actinomycin D from Streptomyces parvulus effectively inhibits Staphylococcus aureus growth. This study reveals its mechanism involves increasing oxidative stress and disrupting energy metabolism in bacteria.

Area of Science:

  • Microbiology
  • Pharmacology
  • Biochemistry

Background:

  • Staphylococcus aureus (S. aureus) is a major pathogen causing significant diseases.
  • The rise of antibiotic resistance necessitates the discovery of novel antibacterial agents.
  • Actinomycin D, derived from Streptomyces parvulus, shows potential as an anti-S. aureus compound.

Purpose of the Study:

  • To investigate the antibacterial activity of actinomycin D against S. aureus.
  • To elucidate the omics profile of S. aureus in response to actinomycin D treatment.
  • To identify the mechanisms underlying actinomycin D's anti-S. aureus effects.

Main Methods:

  • Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays were performed.
  • Bacterial reactive oxygen species (ROS) and hydrogen peroxide (H2O2) levels were quantified.
  • Proteomics and metabolomics analyses were conducted to identify differentially expressed molecules.

Main Results:

  • Actinomycin D demonstrated potent inhibition with MIC of 2 μg/mL and MBC of 64 μg/mL.
  • Treatment with actinomycin D significantly increased ROS and H2O2 levels in S. aureus.
  • Integrated omics data revealed five key pathways related to energy metabolism and oxidative stress disruption.

Conclusions:

  • Actinomycin D exhibits significant antibacterial activity against S. aureus.
  • The anti-S. aureus mechanism involves inducing oxidative stress and impairing energy metabolism.
  • This research provides novel insights into actinomycin D's action, aiding in the development of new antibacterial strategies.
Keywords:
Staphylococcus aureusStreptomyces parvulusactinomycin Dproteomic and metabolomic profiles

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