Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Concise Review Evaluating the Safety and Effectiveness of Emergency General Surgery in Cancer Patients.

The Journal of surgical research·2026

Same author

Temporally Delayed Deployment of Photo-Responsive Liquid Crystal Polymer Networks Toward Neural Interfaces.

Advanced healthcare materials·2026

Same author

Underscoring the Effects of Proton Radiation on Neuronal Function in 2D and 3D In Vitro Cortical Tissue Models.

Radiation research·2026

Same author

Modular Tracking System for Treadmill-Based Rodent Experiments.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025

Same author

Piezo1 and Piezo2 Ion Channels in Neuronal and Astrocytic Responses to MEA Implants in the Rat Somatosensory Cortex.

International journal of molecular sciences·2025

Same author

Type I IFNs enhance human dorsal root ganglion nociceptor excitability and induce TRPV1 sensitization.

JCI insight·2025

Same journal

Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

Micromachines·2026

Same journal

Femtosecond Laser Texturing of Wood Coatings with Bio-Based Epoxy and Wax Additives for Enhanced Hydrophobicity.

Micromachines·2026

Same journal

Engineering of Optoelectronic Devices for Renewable Energy Applications.

Micromachines·2026

Same journal

Phase Transformation and Electrochemical Behavior of Hexagonal TiO<sub>2</sub> Nanotubes Under Different Annealing Temperatures and Heating Rates.

Micromachines·2026

Same journal

Process Optimization and Predictive Modeling of Femtosecond Laser Precision Milling for Commercial PMMA Slices.

Micromachines·2026

Same journal

A Hybrid Preprocessing Multi-Objective Surrogate Model for Thermal MEMS Actuators.

Micromachines·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 12, 2025

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Published on: August 17, 2012

Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System.

Rahul Atmaramani^1,2, Srivennela Veeramachaneni^1,2, Liz Valeria Mogas^1,2

¹Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX 75080, USA.

|November 27, 2021

Summary

This summary is machine-generated.

Peripheral axons of dorsal root ganglion (DRG) neurons play a key role in pain signaling. This study shows microfluidic systems can isolate DRG axons for studying pain mechanisms.

Keywords:

axonal stimulation dorsal root ganglia drug discovery maladaptive plasticity microfluidics nociception

More Related Videos

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Published on: March 9, 2018

Related Experiment Videos

Last Updated: Oct 12, 2025

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Published on: August 17, 2012

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection

Published on: March 9, 2018

Area of Science:

Neuroscience
Molecular Biology
Physiology

Background:

Peripheral axons of dorsal root ganglion (DRG) neurons transmit pain signals.
Axonal plasticity is implicated in pathological pain states.
Nociceptive axons can locally translate mRNA, independent of the cell body.

Purpose of the Study:

To investigate sensory neuron sensitization in isolated axonal structures.
To demonstrate the utility of microfluidic systems for studying pain signaling components.

Main Methods:

Utilized microfluidic systems to compartmentalize DRG axon-like structures.
Treated isolated axons with Interleukin-6 (IL-6) and capsaicin to induce sensitization.
Examined cellular responses within the isolated axonal compartments.

Main Results:

Successfully isolated and maintained DRG axon-like structures in microfluidic channels.
Demonstrated that IL-6 and capsaicin can sensitize these isolated axonal structures.
Provided evidence for localized signaling events within the axons.

Conclusions:

Microfluidic systems are effective for isolating and studying DRG axon function in vitro.
This approach facilitates research into the mechanisms of axonal sensitization in pain pathways.
Lays foundation for complex in vitro models of pain signaling for drug discovery.