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Pyrrole Plasma Polymer-Coated Electrospun Scaffolds for Neural Tissue Engineering
Diana María Osorio-Londoño1,2,3, José Rafael Godínez-Fernández2, Ma Cristina Acosta-García4
1Biomedical Engineering Postgraduate Program, Universidad Autónoma Metropolitana, Iztapalapa, Mexico City 09340, Mexico.
Polymers
|November 27, 2021
Summary
Aligned scaffolds coated with plasma-synthesized pyrrole polymer promote neural cell growth and survival. These biocompatible materials show promise for neural tissue engineering applications, guiding neurite extension without biochemical cues.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Neuroscience
Background:
- Three-dimensional substrates are crucial for neural tissue engineering, supporting cell anchorage and development.
- Electrospun scaffolds offer a promising platform for creating suitable microenvironments for neural cells.
Purpose of the Study:
- To fabricate and characterize fibrillar scaffolds coated with plasma-synthesized pyrrole polymer for neural tissue engineering.
- To evaluate the biocompatibility and neural cell support capabilities of these novel scaffolds.
Main Methods:
- Fabrication of randomly and aligned-oriented electrospun fiber scaffolds coated with doped and undoped plasma-synthesized pyrrole polymer.
- Characterization using infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy.
- Assessment of neural cell adhesion, infiltration, survival, and neurite outgrowth using MTT assays and electron microscopy.
Main Results:
- Scaffolds exhibited a porous fibrillar micrometric structure supporting neural cell adhesion, infiltration, and survival.
- Undoped plasma pyrrole polymer-coated aligned scaffolds promoted neurite-like cell structure elongation without biochemical stimuli.
- MTT assays confirmed the biocompatibility of the composite materials.
Conclusions:
- Plasma-synthesized pyrrole polymer-coated aligned scaffolds are biocompatible and support neural cell adhesion and survival.
- These scaffolds demonstrate potential as permissive substrates for neural tissue engineering, guiding neurite extension.
- The developed materials represent a promising advancement for neural tissue engineering applications.
