Using Crop Databases to Explore Phenotypes: From QTL to Candidate Genes

Seeds, especially those of certain grasses and legumes, provide the majority of the protein and carbohydrates for much of the world's population. Therefore, improvements in seed quality and yield are important drivers for the development of new crop varieties to feed a growing population. Quantitative Trait Loci (QTL) have been identified for many biologically interesting and agronomically important traits, including many seed quality traits. QTL can help explain the genetic architecture of the traits and can also be used to incorporate traits into new crop cultivars during breeding. Despite the important contributions that QTL have made to basic studies and plant breeding, knowing the exact gene(s) conditioning each QTL would greatly improve our ability to study the underlying genetics, biochemistry and regulatory networks. The data sets needed for identifying these genes are increasingly available and often housed in species- or clade-specific genetics and genomics databases. In this demonstration, we present a generalized walkthrough of how such databases can be used in these studies using SoyBase, the USDA soybean Genetics and Genomics Database, as an example.