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Plasmonic Nanoparticle Conjugation for Nucleic Acid Biosensing.

Amogha Tadimety1, Yichen Zhang1, John H Molinski1

  • 1Laboratory of Clinical Genomics and Advanced Technology, Department of Pathology and laboratory Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces gold nanorods with peptide nucleic acid probes for detecting circulating tumor DNA (ctDNA) in liquid biopsies. This method offers rapid, amplification-free detection of ctDNA for diagnostics.

Keywords:
BiosensorCirculating tumor DNAGold nanorodLiquid biopsyLocalized surface plasmon resonanceNanosensorPeptide nucleic acidPlasmonic

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Circulating tumor DNA (ctDNA) is a valuable biomarker for non-invasive cancer detection and monitoring via liquid biopsy.
  • Current ctDNA detection methods can be complex and time-consuming, necessitating the development of rapid and sensitive techniques.
  • Plasmonic nanoparticles offer unique optical properties suitable for sensitive biosensing applications.

Purpose of the Study:

  • To detail the development and application of gold nanorods conjugated with peptide nucleic acid (PNA) probes for sequence-specific ctDNA detection.
  • To present a method for rapid, amplification-free quantification of ctDNA in solution using a plasmonic nanosensor.
  • To provide a resource for researchers interested in utilizing plasmonic gold nanoparticles for solution-based sensing.

Main Methods:

  • Conjugation of peptide nucleic acid (PNA) probes to gold nanorods.
  • Design of PNA probes for sequence-specific hybridization to target ctDNA sequences.
  • Utilizing the optical properties of gold nanorods for amplification-free ctDNA detection and quantitation.

Main Results:

  • Demonstrated successful conjugation of PNA probes to gold nanorods.
  • Achieved sequence-specific detection of ctDNA using the developed nanosensor.
  • Quantified ctDNA levels with a rapid, amplification-free approach.

Conclusions:

  • Gold nanorod-PNA conjugates provide a promising platform for sensitive and specific ctDNA detection.
  • This approach facilitates rapid, amplification-free liquid biopsy analysis for cancer diagnostics and prognostics.
  • The presented methodology can be adapted for various ctDNA detection applications.