Influence of oxygen on the vinyl acetate elimination pathway and microbial community structure of methanogenic sludge

Methanogenic-aerobic coupled processes were used to biological degradation of vinyl acetate (VA) to provide evidence of oxygen role for their complete elimination from different angles. First, physiological characterization of a continuous methanogenic-aerobic reactor fed by VA and glucose (G) showed that by adding G, the VA got 100% hydrolyzed to acetate, and then, by adding 1 mg·L^-1 ·d^-1 of dissolved oxygen (DO), this acetate got methanized by 40% and aerobically mineralized by 60%. Second, batch assays in the presence and absence of sodium azide suggest that VA at different concentrations was eliminated by both anaerobic and aerobic metabolic pathways, because without azide and in the presence of 1 mg DO·L^-1 increased methane and carbon dioxide formation rates at 80% and 75%, respectively. Finally, microbial population dynamics analysis of the reactor by DGGE-sequencing highlighted that Brevibacillus agri (aerobic) and Methanosarcina barkeri (anaerobic) were identified as responsible for VA elimination by up to 98.6%. PRACTITIONER POINTS: Vinyl acetate is removed by simultaneous methanation and aerobic respiration. Methanosarcina barkeri and Brevibacillus agri removed up to 99% of vinyl acetate. DO and VA have a selective effect on the metabolism and population dynamics.