Performance of different microalgae-fungi-bacteria co-culture technologies in photosynthetic and removal performance in response to various GR24 concentrations

The purification effect of two different microalgae-fungi-bacteria symbiosis technologies on biogas and biogas slurry was studied to determine the best symbiosis treatment technology and the suitable concentration of GR24. The results showed that the purification effect of biogas slurry in Chlorella vulgaris-Ganoderma lucidum-endophytic bacteria (S395-2) symbiont co-culture system was better than that of the biogas slurry in Scenedesmus obliquus-Pleurotus ostreatus-S395-2 symbionts. Following 10^-9 M GR24 treatment, Chlorella vulgaris-Ganoderma lucidum-S395-2 symbionts had elevated mean daily production rate and growth rate by 1.92 and 1.46 folds in comparison with blank group. After adjusting the GR24 level within the range of 10^-9 M-10^-7 M, Ganoderma lucidum-assisted Chlorella vulgaris-S395-2 attained higher maximal removal rates for TN, COD, CO₂, and TP by 10.78%, 14.62%, 3.86%, and 9.07%, respectively, compared to the rates when GR24 was not added.