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Microfluidic paper-based chip for parathion-methyl detection based on a double catalytic amplification strategy
Shuhuai Li1, Chaohai Pang2, Xionghui Ma2
1Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, 571101, China. happylishuhuai@163.com.
A new electrochemical chip uses a dual catalytic amplification strategy with Fe3O4 nanozyme-supported carbon quantum dots and silver MOFs for highly sensitive parathion-methyl (PM) detection. This method achieves a low detection limit and is effective in real-world samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Environmental Science
Background:
- Rapid and sensitive detection of insecticides like parathion-methyl (PM) is crucial for food safety and environmental monitoring.
- Existing methods often lack the required sensitivity and selectivity for trace-level insecticide detection.
Purpose of the Study:
- To develop a novel electrochemical microfluidic paper-based chip for the highly sensitive and selective detection of parathion-methyl (PM).
- To utilize a dual catalytic amplification strategy combining Fe3O4 nanozyme-supported carbon quantum dots and silver terephthalate metal-organic frameworks (Fe3O4/C-dots@Ag-MOFs).
Main Methods:
- Synthesis of Fe3O4/C-dots@Ag-MOFs via a hydrothermal method.
- Fabrication of a molecularly imprinted polymer (MIP) on Fe3O4/C-dots@Ag-MOFs using PM as a template.
- Modification of a microfluidic paper-based chip's reaction zone with MIP/Fe3O4/C-dots@Ag-MOFs for electrochemical detection.
Main Results:
- The developed chip demonstrated a low detection limit of 1.16 × 10^-11 mol L^-1 for PM.
- The method achieved high sensitivity and selectivity due to the dual catalytic amplification and MIP.
- Successful determination of PM in agricultural and environmental samples with recovery rates from 82.7% to 109% and RSD < 5.0%.
Conclusions:
- The integration of a dual catalytic amplification strategy with MIP on a microfluidic paper-based chip offers a powerful platform for sensitive insecticide detection.
- This approach significantly enhances detection sensitivity and selectivity, with potential applications for various analytes.
