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Analysis of the long noncoding RNA profiles of RD and SH-SY5Y cells infected with coxsackievirus B5, using RNA

Peiying Teng1, Heng Yang2, Jing Li1

  • 1Medical School, Kunming University of Science and Technology, No. 727, Southern Jingming Road, Chenggong District, Kunming, 650500, Yunnan, People's Republic of China.

Archives of Virology
|November 28, 2021
PubMed

Insights

Long noncoding RNAs (lncRNAs) are key in Coxsackievirus B5 (CV-B5) infections. This study identified lncRNAs in infected cells, revealing lncRNA-IL12A inhibits viral replication, offering new treatment targets.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Hand, foot, and mouth disease (HFMD) caused by Coxsackievirus B5 (CV-B5) poses a significant neurological risk to infants.
  • Long noncoding RNAs (lncRNAs) are recognized for their crucial roles in virus-host interactions.
  • The specific involvement of lncRNAs in CV-B5 pathogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the expression profiles of lncRNAs in human cells infected with CV-B5.
  • To identify differentially expressed lncRNAs and their potential functions in CV-B5 infection.
  • To explore the therapeutic potential of lncRNAs in managing CV-B5-related diseases.

Main Methods:

  • RNA sequencing was employed to profile lncRNA expression in CV-B5-infected human rhabdomyosarcoma (RD) and SH-SY5Y cells.
  • Bioinformatic analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, were performed.
  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to validate the expression of selected lncRNAs.

Main Results:

  • Significant numbers of upregulated and downregulated lncRNAs were identified in both RD (508 up, 760 down) and SH-SY5Y (792 up, 811 down) cells.
  • The majority of differentially expressed lncRNAs were long noncoding intergenic RNAs (lincRNAs) and antisense lncRNAs.
  • Pathway analysis indicated associations with disease in RD cells and involvement in signaling pathways in SH-SY5Y cells.
  • lncRNA-IL12A was identified as a key player that inhibits CV-B5 replication.

Conclusions:

  • lncRNAs exhibit distinct expression patterns in response to CV-B5 infection in different cell types.
  • lncRNAs represent promising molecular targets for the development of novel therapeutic strategies against CV-B5 infection.
  • lncRNAs may serve as potential biomarkers for distinguishing neurogenic complications associated with CV-B5 infection.

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