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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Updated: Oct 11, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Advances in Non-Coding RNA Sequencing.

Julia Micheel1, Aram Safrastyan1, Damian Wollny1

  • 1RNA Bioinformatics/High Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich Schiller University, 07743 Jena, Germany.

Non-Coding RNA
|November 29, 2021
PubMed
Summary
This summary is machine-generated.

High-throughput sequencing advances have accelerated the discovery of non-coding RNAs (ncRNAs) and their diverse roles. This technology significantly expands our understanding of ncRNA biology in various organisms and human health.

Keywords:
diagnosticsliquid biopsynext-generation sequencingnon-coding RNAtranscriptomics

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Non-coding RNAs (ncRNAs) are abundant and functionally diverse RNA molecules involved in fundamental cellular processes.
  • The study of ncRNAs has evolved significantly since their initial discovery.
  • High-throughput sequencing technologies have revolutionized the identification and functional characterization of ncRNAs.

Purpose of the Study:

  • To review recent methodological advancements in high-throughput sequencing for ncRNA research.
  • To highlight how these advancements have expanded the understanding of ncRNA biology across diverse organisms.
  • To discuss the implications of ncRNA discoveries for human health and disease.

Main Methods:

  • Review of recent literature on high-throughput sequencing technologies and protocols.
  • Analysis of advancements in library preparation and computational biology for ncRNA identification.
  • Synthesis of findings on ncRNA roles across different species.

Main Results:

  • High-throughput sequencing has dramatically increased the discovery rate of novel ncRNAs.
  • Methodological improvements have enhanced the comprehensive analysis of ncRNA repertoires.
  • RNA sequencing has revealed critical roles for ncRNAs in various biological processes and diseases.

Conclusions:

  • Modern sequencing techniques are indispensable tools for exploring ncRNA diversity and function.
  • Understanding ncRNA biology through advanced sequencing is crucial for advancing human health.
  • The field of ncRNA research continues to rapidly evolve, driven by technological innovation.