N₂O emission dynamics along an intertidal elevation gradient in a subtropical estuary: Importance of N₂O consumption

Studying nitrous oxide (N₂O) production and consumption processes along an intertidal elevation gradient can improve the understanding of N₂O dynamics among coastal wetlands. A natural-abundance isotope technique was applied to characterize the processes responsible for N₂O emission in high, middle and low intertidal zones in the Yangtze Estuary. The results showed that N₂O emission rates in high tidal zones (0.84 ± 0.35 nmol g^-1 h^-1) were significantly higher than those in middle (0.21 ± 0.04 nmol g^-1 h^-1) and low tidal zones (0.26 ± 0.05 nmol g^-1 h^-1). Gross N₂O production and consumption rates were greater in high and low tidal zones than in middle tidal zones, whereas N₂O consumption proportions generally increased from high to low tidal zones. N₂O consumption was quite pronounced, implying that N₂O emission in estuarine wetlands accounts for only a small fraction of the total production. Higher degrees of N₂O consumption were the pivotal driver of less N₂O emission in low tidal zones. Bacterial denitrification (>84%) was the dominant pathway, although hydroxylamine (NH₂OH) oxidation/fungal denitrification contributed substantially to N₂O production in high tidal flats. The contribution to N₂O production exhibited a decrease in NH₂OH oxidation/fungal denitrification and an increase in bacterial denitrification with decreasing elevation. Changes in N₂O dynamics along the elevation gradient were affected by carbon and nitrogen substrate availabilities as well as the redox environments. Overall, our findings highlight the importance of N₂O consumption in controlling N₂O emission in intertidal wetlands, especially with higher inundation frequencies and durations.