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A monoclonal antibody reactive with human eosinophils
Blood
|January 1, 1986
Summary
A new antibody, EO-1, specifically targets eosinophils, basophils, and platelets, differentiating them from neutrophils. This unique reactivity makes EO-1 a valuable tool for studying these cell types and their development.
Area of Science:
- Immunology
- Hematology
Background:
- Eosinophils, basophils, and platelets play critical roles in immune responses and hemostasis.
- Existing monoclonal antibodies often lack specificity, cross-reacting with neutrophils.
Purpose of the Study:
- To characterize a novel murine monoclonal antibody, EO-1, for its reactivity profile.
- To determine the potential of EO-1 as a specific diagnostic or research tool.
Main Methods:
- Generation of EO-1, an IgG1 murine monoclonal antibody against human eosinophilic leukemia cells.
- Immunophenotyping of various hematopoietic cells (eosinophils, basophils, platelets, neutrophils, mononuclear cells, myeloid lines) using EO-1.
- Immunoprecipitation and SDS-PAGE analysis of EO-1 antigen from surface-labeled platelets.
Main Results:
- EO-1 reacts with eosinophils, basophils, platelets, and a small subset of mononuclear cells.
- EO-1 does not react with neutrophils or immature myeloid cells.
- Immunoprecipitation identified the EO-1 antigen as a 23,000 molecular weight protein on platelets.
- EO-1 reactivity is consistent with antigen expression on mature and immature eosinophils and basophils in bone marrow.
Conclusions:
- EO-1 exhibits unique specificity for eosinophils, basophils, and platelets.
- Unlike previously described antibodies, EO-1 does not cross-react with neutrophils.
- EO-1 represents a valuable reagent for investigating the function and differentiation of eosinophils, basophils, and platelets.