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Porcine iPSC Generation: Testing Different Protocols to a Successful Application.

Oriol Iborra-Egea1, Daina Martínez-Falguera1, Santiago Roura1,2

  • 1MyoCare Lab, ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), Badalona, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2021
PubMed
Summary

Stem cell therapy shows promise for treating diseases, but requires large animal studies. This chapter details protocols for swine induced pluripotent stem cell (iPSC) reprogramming for preclinical cardiovascular research.

Keywords:
Episomal nucleofection reprogrammingPorcine iPSCRNA reprogrammingRetroviral reprogrammingSendai virus reprogramming

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Stem cell therapy holds significant potential for treating various diseases, including blood cancers, cardiovascular conditions, and neurodegenerative disorders.
  • Advancing stem cell therapeutics to clinical trials necessitates rigorous preclinical evaluations, particularly large animal safety and efficacy studies.
  • Swine models are extensively utilized in cardiovascular research due to their physiological similarities to humans, offering high translational value.

Purpose of the Study:

  • To describe protocols for inducing dedifferentiation of induced pluripotent stem cells (iPSCs) in swine fibroblasts.
  • To outline conditioning treatments that can enhance the stem cell reprogramming process in swine models.
  • To facilitate the preclinical development of stem cell therapeutics for cardiovascular diseases using swine models.

Main Methods:

  • Detailed protocols for the dedifferentiation of swine fibroblasts into iPSCs.
  • Description of various conditioning treatments aimed at improving reprogramming efficiency.
  • Methodologies for assessing the quality and pluripotency of reprogrammed swine iPSCs.

Main Results:

  • Establishment of reliable protocols for generating swine iPSCs from fibroblasts.
  • Identification of conditioning treatments that positively influence the reprogramming efficiency.
  • Characterization of swine iPSCs derived through the described methods.

Conclusions:

  • The presented protocols provide a foundation for generating swine iPSCs, crucial for preclinical cardiovascular research.
  • Optimized reprogramming conditions can accelerate the development of stem cell-based therapies.
  • This work supports the advancement of stem cell therapeutics towards clinical applications in cardiovascular medicine.