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QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification.

Tyler Dang1, Jinbo Wang1,2, Tavia Rucker1

  • 1Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 30, 2021
PubMed
Summary

The QuantiGene Plex assay offers a non-PCR method for detecting and identifying citrus viroids. This high-throughput screening tool works with both purified and crude RNA extracts from citrus plants.

Keywords:
Branched DNABranched amplification assayHigh-throughputLuminex MAGPIX®Multiplex

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Area of Science:

  • Plant Pathology
  • Molecular Diagnostics
  • Agricultural Entomology

Background:

  • Citrus viroids are significant pathogens affecting citrus production worldwide.
  • Accurate and efficient detection methods are crucial for disease management and preventing spread.

Purpose of the Study:

  • To adapt and validate the QuantiGene Plex assay for citrus viroid detection.
  • To establish the QuantiGene Plex assay as a high-throughput screening tool for viroids in citrus.

Main Methods:

  • Utilized the QuantiGene Plex assay, a molecular, non-polymerase chain reaction (PCR)-based multiplex method.
  • Applied the assay to both purified and crude RNA extracts from citrus tissues.

Main Results:

  • The QuantiGene Plex assay demonstrated effectiveness for citrus viroid detection and identification.
  • The method is suitable for high-throughput screening applications.

Conclusions:

  • The QuantiGene Plex assay provides a viable alternative to PCR-based methods for citrus viroid diagnostics.
  • This assay facilitates efficient and scalable screening of citrus for viroid infections.