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An In Silico Detection of a Citrus Viroid from Raw High-Throughput Sequencing Data
Tyler Dang1, Andres Espindola2, Georgios Vidalakis3
1Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
Abstract:
E-probe Diagnostic for Nucleic Acid Analysis (EDNA) is a user-friendly bioinformatic tool that has been adapted for the detection and identification of citrus exocortis viroid (CEVd). Here, we describe the procedures for RNA extraction from citrus tissues, library and sequencing preparation, and the utilization of EDNA Mi-Finder online platform on raw high-throughput sequencing (HTS) data.
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