A two-stage energy tuning strategy via salt and glycine programmed DNA-engineered crystals

To obtain high-quality DNA-engineered crystals at room temperature, a two-stage energy tuning strategy by first adding NaCl and then glycine (Gly) is proposed. The addition of Gly can exquisitely balance the attraction and repulsion energies for crystallization. The state transition induced by energy rather than temperature is significant for a biosystem.