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Specific detection of IMP-1 β-lactamase activity using a trans cephalosporin-based fluorogenic probe

Liqiang Hu1, Runqiu Liu1, Zheng Ma1

  • 1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. xiehexin@ecust.edu.cn.

Chemical Communications (Cambridge, England)
|November 30, 2021
PubMed

Keywords:
Antibiotic ResistanceEnzymatic DetectionFluorescence ImagingDiagnostic Reagents

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...