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Buffer concentration dramatically affects the stability of S-nitrosothiols in aqueous solutions.
Wuwei Li1, Danyang Wang1, Ka Un Lao1
1Department of Chemistry, Virginia Commonwealth University, 1001 W Main St., Richmond, VA, 23284, USA.
Nitric Oxide : Biology and Chemistry
|December 1, 2021
Summary
Buffer concentration critically impacts S-nitrosothiol (RSNO) stability. Optimal buffer levels enhance nitric oxide (NO)-donating compound stability for biomedical applications, preventing premature decomposition.
Area of Science:
- Biochemistry
- Chemical Biology
- Materials Science
Background:
- S-nitrosothiols (RSNOs) are vital nitric oxide (NO)-donating molecules with significant biomedical potential.
- The stability of RSNO solutions is crucial for their therapeutic efficacy and prolonged storage.
- Previous studies have overlooked the influence of buffer concentration on RSNO stability.
Purpose of the Study:
- To investigate the effect of buffer concentration on the stability of S-nitrosothiols (RSNOs).
- To determine optimal buffer conditions for maximizing the stability of S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylpenicillamine (SNAP).
- To provide guidance for formulating stable RSNO solutions for research and therapeutic applications.
Main Methods:
- Experimental analysis of RSNO stability under varying buffer concentrations.
- Density Functional Theory (DFT) calculations to assess RSNO stability at different pH levels.
- Investigation of buffer components' interaction with NO/N2O3.
Main Results:
- Optimal buffer concentration for high GSNO concentrations (50 mM) is near the GSNO concentration itself, balancing pH resistance and minimizing NO scavenging.
- Maximum SNAP stability is achieved in solutions without buffer, as its decomposition-induced pH drop enhances stability.
- Both low and high buffer concentrations can decrease RSNO stability, with excess buffer accelerating decomposition.
Conclusions:
- Buffer concentration is a critical, previously overlooked factor influencing RSNO stability.
- Tailoring buffer concentration to specific RSNOs and concentrations is essential for maximizing solution longevity.
- This study provides crucial insights for the effective formulation of RSNOs for sustained nitric oxide release.
Keywords:
Buffer concentrationDensity functional theoryNitric oxide donorS-nitroso-N-AcetylpenicillamineS-nitrosoglutathioneS-nitrosothiol stabilityMore Related Videos
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