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Updated: Oct 11, 2025
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Published on: September 9, 2011
Characteristics of Human Peripheral Blood γδ T Cells Expanded With Zoledronate
Minsong Kim1, Hyori Kim1, Mina Han1
1Asan Institute for Life Sciences and Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
Background/Aim:
This study aimed to investigate the characteristics of human peripheral blood γδ T cells, which were expanded ex vivo in the presence of zoledronate (ZOL).
Materials And Methods:
Human peripheral blood cells were cultured with IL-2 and IL-15 in the presence or absence of ZOL, which was added as a phospho-antigen, and their phenotypes were assessed by flow cytometry. Expanded γδ T cells were transduced with CD19 CAR vector, and the cytotoxicity was evaluated in vitro and in vivo by flow cytometry.
Results:
Ex vivo expansion did not hamper the expression of activating receptors. Interestingly, ZOL promoted the expression of CD226 (DNAM-1), TRAIL, and FAS-L in the Vδ1 subset of γδ T cells. Expanded γδ T cells containing CD19 CAR+ γδ T cells removed B cell lymphoma cells effectively in vivo.
Conclusion:
γδ T cells could be a promising immunotherapeutic for cancer.
