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Related Experiment Video

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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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Regulatory circuits involving bud dormancy factor PpeDAM6.

Alba Lloret¹, Carles Quesada-Traver¹, Ana Conejero¹

¹Instituto Valenciano de Investigaciones Agrarias, 46113, Moncada, Valencia, Spain.

Horticulture Research

|December 1, 2021

Summary

Dormancy-associated MADS-box (DAM) genes regulate perennial plant dormancy. PpeDAM6 in peach represses bud break, interacting with BASIC PENTACYSTEINE PROTEINs (PpeBPCs) and influencing hormone pathways for growth regulation.

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Area of Science:

Plant Biology
Molecular Genetics
Epigenetics

Background:

DORMANCY-ASSOCIATED MADS-BOX (DAM) genes are crucial for perennial plant dormancy and climate adaptation.
PpeDAM6 in peach (Prunus persica) is a key repressor of bud dormancy release and bud break.
PpeDAM6 expression decreases with winter chilling, linked to H3K27me3 modification.

Purpose of the Study:

Investigate the molecular mechanisms regulating PpeDAM6 activity and its role in plant growth.
Identify proteins interacting with the PpeDAM6 regulatory region.
Determine the function of PpeDAM6 in controlling vegetative growth and hormone homeostasis.

Main Methods:

Identified peach BASIC PENTACYSTEINE PROTEINs (PpeBPCs) interacting with specific DNA motifs.
Performed transient expression experiments to assess PpeBPC1's effect on PpeDAM6 promoter activity.
Generated transgenic European plum (Prunus domestica) overexpressing PpeDAM6.

Main Results:

Discovered three PpeBPCs interacting with GA-repeat motifs in the PpeDAM6 regulatory region.
PpeBPC1 was found to repress PpeDAM6 promoter activity.
Overexpression of PpeDAM6 in plum led to dwarfism, shoot meristem collapse, and altered hormone pathways (jasmonic acid, cytokinin, abscisic acid, gibberellin).

Conclusions:

PpeBPCs are involved in regulating PpeDAM6 expression.
PpeDAM6 plays a significant role in controlling plant vegetative growth and hormone balance.
Hormone homeostasis mechanisms are implicated in PpeDAM6-mediated repression of floral bud dormancy and growth.